Charlie the Unicorn is a 2005 Flash animated comedy short film and viral video created by Jason Steele of independent film company FilmCow in Orlando, Florida. The short follows Charlie, a lethargic and pessimistic unicorn, who is taken by a blue unicorn and a pink unicorn on an adventure to the magical Candy Mountain. However, the journey turns out to be a farce, and Charlie has his kidney stolen by the other two unicorns.

Steele originally created the video as a birthday present for his mother; the video was partially credited to her and was released by her under the username "TypeQueen" on Newgrounds, where it rapidly increased in popularity. The video was later uploaded onto YouTube by Geoff Swanson, where it gained large viewership and continued to increase in popularity, leading to a series of sequels and spinoffs. A merchandising line was later produced, as well as four sequels and a parody series titled Charlie teh Unicron. The first three episodes in the series were released to DVD in 2009 as part of The FilmCow Master Collection.